Documentation

Molecular Biology

This framework allows users to corvert HLA types from Low or Medium resolution to High resolution.

Fields:
Population: Ethnic groups from the American population. This work was performed by Maiers et al. [1] where he describes the allele frequencies for HLA alleles in the US population.
Possible Options:
  • AFA: African Americans.
  • API: Asians.
  • EUR: Caucasians.
  • HIS: Hispanics.
 Locus: It can be A, B, C for class I HLA; and DRB1, DQB1 for class II HLA. This field corresponds to the kind of HLA Molecule typed.

Allelic Group: 2 digits – correspond to low resolution in Molecular Biology. Example: 02 to HLA-A*, 07 to HLA-B*.

NMDP Code: code of letters that represents a group of alleles possible for the patient in a specific allelic group. Together locus, allelic group and NMDP code correspond to medium resolution. Example: A*24:FPDF.

Linkage Molecular Biology:

In this section, the user can easily see class II linkage (DRB345, DQA1, DQB1) to specific DRB1 [2] .

DRB1 types can be informed in low, medium or high resolution (Molecular Biology). If high resolution is available, low or medium resolution is not considered.

Optionally, the user can inform DQB1 types (low, medium or high resolution – Molecular Biology) in order to separate genotypes in groups according presence or absence of DQB1 types.

Linkage Serology:

In this section, the user can easily see class II linkage (DRB345, DQA1, DQB1) to a specific DRB1 [2] .

DRB1 types should be informed in serology (example: DR11, DR15). Optionally, the user can inform DQB1 types (serology: DQ7, DQ2) in order to separate genotypes in groups according to presence or absence of DQB1 types.

Database Converter:

In this section you can upload your database (click here to download the model) and convert all from low or medium resolution to high resolution (Molecular Biology).

Example of use:

  1. Download the example. [Click here].
  2. Go to main menu Database Converter.
  3. Choose the "Population". Ex.: 'EUR'.
  4. Choose the "Out model": in this field you can choose between "Old" (Ex.: 0202) and "New" (Ex.: 02:02) nomenclatures for generate the output file Ex.: 'New Nomenclature'.
  5. Upload the example file.
  6. Click on "Send" button.
  7. Save the generated file!



References and notes

  1. MAIERS, M. et al. High-resolution HLA alleles and haplotypes in the United States population. Hum Immunol [S.I.], v. 68, n. 9, p. 779-88, Sep 2007
  2. BURDETT, L. et al. DRB-DQB1 diversity in the analysis of 4727 donors typed by SBT. . Human Immunology [S.I.], v. 64, n. 10, p. S6, 2003.